Description The Department of Critical Care Medicine in the Cumming School of Medicine invites applications for a Laboratory Technician . This Full-time Fixed Termposition is for approximately 1 year (based on length of grant funding), with the possibility of extension. This position reports to the CCMT Program Manager and supports a translational research and development program focused on live biotherapeutic products (LBPs) derived from the human microbiome. The technician will perform routine tasks of moderate to high complexity and contribute to coordinating laboratory operations. Examples include (but are not limited to) anaerobic culture and isolation of commensal bacteria, preparation of media, cryopreservation and biobanking of isolated, nucleic acid extraction and routine bacteriological characterization Culture, isolate, and maintain strictly anaerobic and facultative bacteria from human stool and derived materials, including work in an anaerobic chamber and preparation of reduced and selective media Perform molecular biology procedures including genomic DNA and RNA extraction, PCR, gel electrophoresis, plasmid preparation, restriction digestion and ligation, transformation, and sequencing library preparation Carry out bacterial cloning and genetic manipulation (e.g. plasmid construction, conjugation or electroporation-based transformation, mutant construction and verification) as directed Support cell banking activities, including cryopreservation, inventory management, and accurate registration of strains within the CCMT strain registry and sample tracking systems Maintain detailed records of experimental protocols, raw data, and analyses to support publications, regulatory submissions, and quality documentation Maintain and monitor laboratory equipment (anaerobic chamber, incubators,



centrifuges, freezers, etc) including routine performance checks and service coordination Draft, maintain, and follow SOPs and monitor key laboratory practices, inventory, and consumable ordering Provide aid in the training and supervision of students and other members of the laboratory Adhere to all University of Calgary policies and regulatory requirements, and to biosafety standards including the handling of human-derived material and Containment Level Bachelors degree in microbiology, biochemistry, molecular biology or a related discipline is required. A masters degree or equivalent laboratory experience is an asset Demonstrated hands-on experience in bacteriology, including aseptic technique, media preparation, culture, isolation and enumeration, is required Demonstrated experience in molecular biology techniques (e.g. nucleic acid extraction, PCR/qPCR, electrophoresis) is required Experience with bacterial cloning and genetic manipulation (plasmid construction, transformation, mutant generation and verification) is strongly preferred Experience with anaerobic culture techniques and/or experience in anaerobic chamber operation is an asset Experience with gut microbiome research, commensal or fecal-derived isolated, or live biotherapeutic product development is an asset Familiarity with sequencing workflows (16s rRNA amplicon and/or whole-genome sequencing library preparation)



is an asset Exposure to GMP, GLP, or other quality-controlled environments, including SOP-driven documentation, is an asset Strong record-keeping and data management skills, with careful attention to detail Must be able to work effectively as part of a team and to manage independent tasks with limited supervision Intermediate to advanced skills in the Microsoft Office Suite (Word, Excel, PowerPoint) Knowledge of University or Industry research environments is an asset WHMIS and Biosafety training (or willingness to complete upon hire) This position is part of the AUPE bargaining unit, and falls under the Technical Job Family, Phase 1. It is a top research university and one of the highest-ranked universities of its age. Founded in 1966, its 36,000 students experience an innovative learning setting, made rich by research, hands-on experiences and entrepreneurial thinking. It is Canada's leader in the creation of start-ups Start something today at the University of Calgary. The University of Calgary has launched an institution-wide Indigenous Strategy ii' taa'poh'to'p committing to creating a rich, vibrant, and culturally competent campus that welcomes and supports Indigenous Peoples, encourages Indigenous community partnerships, is inclusive of Indigenous perspectives in all that we do. The university's commitment to the Indigenous Strategy is evident through the oversight of 18 Elders serving on the Circle of Advisors and the many unit-based circles working towards implementation of the strategy including the Faculty Advisory Circle and teams such as the Office of Indigenous Engagement, the Writing Symbols Lodge, and the Indigenous Research Support Team. Many Indigenous-based

📌 Laboratory Assistant- Permanent Full Time (Calgary)
🏢 University of Calgary
📍 Calgary