Research Project Regulation of neutral lipid storage and production in mammalian cells Department/Unit Department/Unit Pathology Posting Number Posting Number S331-26 Employee Group Grant-Paid (PSAC Local 86001) Duration of Contract (if applicable) Duration of Contract (if applicable) 9 months Employment Type Full Time Full-time Equivalency (FTE) Full-time Equivalency (FTE) 1.0 Dalhousie University Dalhousie University is Atlantic Canada’s leading research-intensive university and a driver of the region’s intellectual, social and economic development. Located in the heart of Halifax, Nova Scotia, with an Agricultural Campus in Truro/Bible Hill, Dalhousie is a truly national and international university, with more than half of our almost 21,000 students coming from outside of the province. Our 6,000 faculty and staff foster a vibrant, purpose-driven community, that celebrated 200 years of academic excellence in 2018.

Under the Principal Investigator, the research will have broad aims focusing on the hydrolysis of TAG in lipid droplets and cholesterol synthesis. Elements of the research will examine the translational control of cholesterol biosynthetic enzymes and the post-translational regulation of lipases. Reporting to the Principal Investigator, the Research Technician will conduct cell-based biochemical assays and microscopy to support two projects examining neutral lipid metabolism.

Maintain, passage, treat and experimentally manipulate mammalian cell lines using aseptic technique and approved protocols. Collect and preserve cells and subcellular samples for biochemical, imaging and downstream analyses. Perform biological replicates and appropriate controls, monitor assay quality and repeat experiments when required.

Measure protein S-palmitoylation using acyl-biotin exchange ( ABE ) and metabolic labelling/click-chemistry workflows. Prepare cell lysates and perform protein quantification, affinity enrichment,



protein electrophoresis ( SDS - PAGE ), immunoblotting and quantitative analysis. Prepare live or fixed mammalian-cell samples and acquire conventional fluorescence and fluorescence lifetime microscopy ( FLIM - FRET ) data.

Analyze fluorescence lifetimes and FRET responses and quantify lipid droplet abundance, morphology and protein localization using appropriate software. Maintain accurate, timely and traceable laboratory records, including raw data, calculations, isotope records and protocol deviations. Analyze experimental data, prepare summary graphs and figures, and present progress at laboratory meetings.

In addition, they will be required to handle Biosafety Level 2 cell lines, viruses, bacteria, and radioisotopes, which will require PPE and may be considered a hazard or danger to the incumbenton a daily basis. Undergraduate degree in cell biology, biochemistry, molecular biology or a closely related discipline plus at least one year of related research-laboratory experience, including independent hands-on mammalian cell culture (or equivalent combination of training and experience). Demonstrated hands-on experience measuring protein S-palmitoylation using acyl-biotin exchange or metabolic labelling/click chemistry;

Experience with protein quantification, affinity enrichment, SDS - PAGE , immunoblotting, fluorescence microscopy and quantitative image analysis. Required institutional biosafety, WHMIS , laboratory safety and radiation-safety training must be completed or maintained as a condition of employment.

Assets





Master of Science in cell biology, biochemistry, molecular biology or a closely related discipline.

Experience with super-resolution and analysis of FLIM - FRET data. Our total compensation package includes a voluntary RRSP , health and dental plans, a wellness allowance, an employee and family assistance program and a tuition assistance program. Non-probationary bargaining unit employees ( PSAC Local 86001) are given priority consideration.

All other applications will be considered when there is no qualified candidate from the bargaining unit. Full time students currently enrolled at an educational institution are not eligible for regular Grant-Paid positions.

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University commits to achieving inclusive excellence through continually championing equity, diversity, inclusion, and accessibility. The university encourages applications from Indigenous Peoples of Turtle Island (especially Mi'kmaq), persons of Black/African descent (especially African Nova Scotians), and members of other racialized groups, persons with disabilities, women, persons identifying as members of 2SLGBTQIA+ communities, and all candidates who would contribute to the diversity of our community. In accordance with our Employment Equity Policy, preference will be given in hiring processes to candidates who self-identify as members of one or more of the equity-deserving groups listed above.

For more information, including details related to our Employment Equity Policy and Plan and definitions of equity-deserving groups please review our Employment Equity information.If you require any support for the purpose of accommodation, such as technical aids or alternative arrangements, please let us know of these needs and how we can be of assistance. Our complete Accommodation Policy can be viewed here . Required fields are indicated with an asterisk (*).

📌 Research Technician - Per Diem (Halifax Regional Municipality)
🏢 Dalhousie University
📍 Halifax Regional Municipality