Description The Office of the Associate Dean Research in the Cumming School of Medicine invites applications for a Spatial & Single Cell Omics Technician. This Full time Fixed Term position is for approximately 12 months (based on length of grant funding), with the possibility of extension. This is a hands-on technical position in a fee-for-service core facility. Work involves multiple concurrent projects at different stages, strict chain-of-custody and documentation requirements, and careful handling of limited or irreplaceable research samples. The technologist selects and applies established procedures according to sample type, platform requirements and project specifications. Judgment is required when assessing sample suitability, interpreting quality-control results, adjusting work within validated parameters and determining when a deviation requires consultation with the ASOC Manager, scientific lead or instrument vendor. The role works closely with ASOC staff, researchers, trainees, bioinformatics personnel, other CSM core facilities and external technical support. The incumbent explains sample requirements, provides task-specific instruction on approved procedures and contributes observations to workflow improvements. Regular hours are normally Monday to Friday. Occasional early morning, evening or weekend work may be required to complete time-sensitive protocols, monitor instrument runs or respond to equipment issues. The laboratory environment includes prolonged standing or sitting, repetitive pipetting, handling of biological specimens and chemicals, and use of appropriate personal protective equipment. Reporting to the Director, Strategic Projects. Summary of

Key Responsibilities
Spatial Omics and Single-cell Omics Workflows
- Receive, verify, document and track tissue, cell,



DNA and RNA samples according to ASOC procedures and project specifications
- Assess sample and tissue suitability using established acceptance criteria, microscopy and nucleic acid quality-control methods; document findings and consult on samples that fall outside specifications
- Perform established spatial transcriptomics, spatial proteomics and single-cell
- Perform nucleic acid extraction, quantification, normalization, amplification and library preparation using manual and automated methods
- Maintain sample identity, orientation, metadata and chain-of-custody records throughout processing
- Work with ASOC staff and users to ensure that sequencing plans are appropriate for the spatial assay, library structure, required read configuration, expected data volume and downstream analysis pathway
- Transfer spatial and single-cell omics outputs to approved storage and analysis environments, verify file completeness and maintain accurate data-delivery records Next-Generation Sequencing
- Prepare, normalize and pool sequencing libraries using validated protocols and project-specific index information
- Perform sequencing library quality control using established electrophoretic, fluorometric and/or qPCR-based methods, and confirm readiness for pooling and sequencing according to defined acceptance criteria
- Create and verify sample sheets, run configurations and instrument workflows, including index orientation,



read structure and demultiplexing requirements
- Set up, operate and monitor the Illumina NovaSeq X series and other high-throughput sequencing instruments used by ASOC or partner core facilities
- Perform routine instrument cleaning, maintenance, calibration and operational checks; maintain service records and coordinate technical support when required
- Transfer sequencing output to approved storage and analysis environments, verify file completeness and maintain accurate data-delivery records
- Technical Quality and Troubleshooting
- Review quality-control results at defined checkpoints and document decisions, deviations and corrective actions
- Troubleshoot routine sample, reagent, assay, software and instrument problems using established decision paths, prior run information and vendor guidance
- Investigate discrepancies in collaboration with the ASOC Manager and elevate issues that fall outside validated limits or delegated authority
- Contribute technical observations and test results to validation, verification and method-comparison activities led by senior staff User Support and Training
- Explain ASOC sample requirements, submission processes, workflow stages and routine technical limitations to researchers and trainees
- Provide task-specific demonstrations and hands‑on instruction in approved procedures, instrument use and laboratory documentation
- Communicate sample concerns, technical findings, delays and next steps clearly and promptly, referring project‑design or interpretive questions to the appropriate scientific or bioinformatics lead Laboratory Operations and Documentation
- Maintain complete records in laboratory information, project‑tracking, inventory and billi

📌 Spatial & Single Cell Omics Technician, Office of the Associate Dean Research (Calgary)
🏢 University of Calgary
📍 Calgary