Research Technician III - Donald K. Johnson Eye Institute Contract
Department: Research
Compensation: CAD 37.47 - CAD 46.84 - hourly
UHN is Canada’s #1 hospital and the world’s #1 publicly funded hospital. With 10 sites and more than 44,000 TeamUHN members, UHN consists of Toronto General Hospital, Toronto Western Hospital, Princess Margaret Cancer Centre, Toronto Rehabilitation Institute, The Michener Institute of Education and West Park Healthcare Centre. As Canada's top research hospital, the scope of biomedical research and complexity of cases at UHN have made it a national and international source for discovery, education and patient care. UHN has the largest hospital-based research program in Canada, with major research in neurosciences, cardiology, transplantation, oncology, surgical innovation, infectious diseases, genomic medicine and rehabilitation medicine. UHN is a research hospital affiliated with the University of Toronto.
UHN’s vision is to build A Healthier World and it’s only because of the talented and dedicated people who work here that we are continually bringing that vision closer to reality.
Union: Non-union
Number of vacancies: 1
New or Replacement Position: New
Site: Toronto Western Hospital
Department: Donald K. Johnson Eye Institute
Reports to: Principal Investigator
Salary Range : $37.47 - $46.84 per hour
Hours: 37.5 hours per week
Status: Temporary Full time
Closing Date: November 4, 2026
Position Summary
The Research Technician III will play a key role in supporting innovative neuroscience and vision research within the Principal Investigator’s laboratory at the Donald K. Johnson Eye Institute and Krembil Research Institute. Working as part of a multidisciplinary research team,



the incumbent will maintain and characterize human multipotent and pluripotent stem cell cultures, support the development of advanced 3D organoid models of the brain and eye, and perform a range of molecular and cellular biology techniques to advance studies of neurological and retinal disease.
Duties
Maintaining cell lines (cultured and primary), including human multipotent and pluripotent stem cell cultures, using standard sterile techniques
Conduct experiments to support ongoing lab research projects (Western blots, qRT-PCR, immunocytochemistry and immunohistochemistry)
Management of tissue culture facilities, ordering supplies, lab management
Support the day-to-day operations of the laboratory, including stock preparation, supplies ordering, and general lab maintenance
Assist with biosafety compliance, including biological/chemical inventory management
Help develop standard operating procedures and protocols for lab-related activities
Attend and present data in weekly lab meeting discussions, institutional research seminars, and research conferences
Some administrative support to the PI At minimum, a Bachelors degree in biological sciences, or equivalent discipline is required
At minimum, 6 years of related experience is required
Prior experience with multipotent and pluripotent stem cell cultures is required




3D organoid culture experience is preferred
Prior experience with standard laboratory protocols for cell culture, Western blot, qRT-PCR, DNA electrophoresis and genotyping, molecular cloning
Prior experience with primary cell isolation from tissue specimens (animals or human) is an asset
Viral transduction and genetic engineering / gene-editing (e.g., CRISPR/Cas9) experience is an asset
RNA sequencing and bioinformatics experience is an asset
Willing to rapidly learn new techniques and skills with training
Capacity to work independently with minimal supervision
Highly-organized and able to prioritize effectively
Able to communicate and interact with staff, students, colleagues in a professional manner and daily basis (on-site)

Why Join UHN?
Competitive offer packages
Government organization and a member of the Healthcare of Ontario Pension Plan (HOOPP https://hoopp.com/ )
Close access to Transit and UHN shuttle service
A flexible work environment
Opportunities for development and promotions within a large organization
Additional perks (multiple corporate discounts including: travel, restaurants, parking, phone plans, auto insurance discounts, on-site gyms, etc.)
Current UHN employees must have successfully completed their probationary period, have a good employee record along with satisfactory attendance in accordance with UHN's attendance management program, to be eligible for consideration.
UHN is an equal opportunity employer committed to an inclusive recruitment process and workplace. Requests for accommodation can be made at any stage of the recruitment process. Applicants need to make their requirements known.

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📌 Research Technician III - Donald K. Johnson Eye Institute (Ontario)
🏢 UHN Research
📍 Ontario